Assays

Assay to measure metabolites of yeast saccharomyces cerevisiae under different nutrient limitations

Limiting Nutrients:
ethanol, nitrogen, glucose, phosphorus, carbon, sulfur, sulphur

This data is public data taken from the BioInvestigation Index. (http://www.ebi.ac.uk/bioinvindex/study.seam?studyId=BII-S-1)

Contributor: Katy Wolstencroft

Assay type: Metabolite Profiling

Technology type: Mass Spectrometry

Snapshots: No snapshots

Assay to measure protein expression in yeast saccharomyces cerevisiae under different nutrient limitations

Limiting Nutrients:
ethanol, nitrogen, glucose, phosphorus, carbon, sulfur, sulphur

This data is public data taken from the BioInvestigation Index. (http://www.ebi.ac.uk/bioinvindex/study.seam?studyId=BII-S-1)

Contributor: Katy Wolstencroft

Assay type: Protein Expression Profiling

Technology type: Mass Spectrometry

Snapshots: No snapshots

Assay to measure gene expression of Saccharomyces cerevisiae (yeast) under different nutrient limitations

Limiting Nutrients:
ethanol, nitrogen, glucose, phosphorus, carbon, sulfur, sulphur

This data is public data taken from the BioInvestigation Index.

(http://www.ebi.ac.uk/bioinvindex/study.seam?studyId=BII-S-1)

Contributor: Katy Wolstencroft

Assay type: Transcriptional Profiling

Technology type: Microarray

Snapshots: No snapshots

An assay to measure exttracellular glycerol using HPLC

Contributor: Katy Wolstencroft

Assay type: Metabolite Profiling

Technology type: HPLC

Snapshots: No snapshots

A kinetic model of the glycerol synthesis pathway has been constructed. Kinetic parameters were collected from published values. Maximal enzyme activities and intracellular effector concentrations were determined experimentally.

Contributor: Katy Wolstencroft

Biological problem addressed: Metabolism

Snapshots: No snapshots

Intracellular metabolite concentrations were determined enzymatically by measuring the oxidation or reduction of NADH or NAD+, respectively, at 340 nm in a Beckman Coulter DU640 spectrophotometer

No description specified

Contributor: Katy Wolstencroft

Assay type: Glucose Pulse

Technology type: NMR

Snapshots: No snapshots

Assay for transcriptional profiling time course in yeast treated with rapamycin

This is public data from the BioInvestigation Index (http://www.ebi.ac.uk/bioinvindex/study.seam?studyId=BII-S-2)

Detailed glycolytic model in Lactococcus lactis

Contributor: Katy Wolstencroft

Biological problem addressed: Metabolic Network

Snapshots: No snapshots

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