Data files

Saturation of PGK with 3PG

Kinetic characterisation of Phosphoglycerokinase. Measures enzyme reaction rate with increasing concentration of ATP

GAP concentrations were measured over time at 70C.

DHAP concentrations were measured over time at 70C.

A time simulation of the mathematical model is shown together with the experimental data to which the model was fitted.

A time simulation of the mathematical model is shown together with the experimental data to which the model was fitted.

Model simulation and experimental data for DHAP and GAP saturation of FBPA/ase

Model simulation and experimental data for ADP, ATP, 3PG and BPG saturation of PGK

Model simulation and exp data for TIM kinetics, GAP and DHAP saturation, and PEP inhibition.

The purified enzymes, PGK, GAPDH, TPI and FBPAase were incubated at 70 C en conversion of 3PG to F6P was followed