SOPs

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Cloning and heterologous expression of gluconeogenic enzymes in E. coli

Preparation of cell free extracts of the recombinant E. coli strains expressing the respective S. solfataricus enzymes.

The recombinant enzymes were purified from the cell free extracts of E. coli expressing the respective enzymes.

A stationary-phase culture (10ml) was used to inoculate the fermenter vessel.

Taken from ArrayExpress:
http://www.ebi.ac.uk/microarray-as/ae/browse.html?keywords=E-MEXP-115

The procedure describes the preparation of fluorescent DNA probes from human mRNA or total
RNA.

Creator: Olga Krebs

Contributor: Olga Krebs

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