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Mathematical model for the analysis of carbon loss of BPG, GAP and DHAP in reconstituted system of S. solfataricus at 70C.
SEEK DEMO ID: http://demo.seek4science.org/assays/26
Modelling Analysis
Projects: SulfoSys
Investigation: Central Carbon Metabolism of Sulfolobus solfataricus
Study: Model gluconeogenesis
Biological problem addressed: Model Analysis Type
Organisms: Sulfolobus solfataricus : P2 (wild-type / wild-type)
Data:

Views: 777
Created: 2nd Feb 2013 at 14:21
Last updated: 26th Oct 2017 at 11:57

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- People (1)
- Projects (1)
- Investigations (1)
- Studies (1)
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- Publications (1)
Projects: PSYSMO, MOSES, SysMO DB, SysMO-LAB, SulfoSys
Institutions: Manchester Centre for Integrative Systems Biology, University of Manchester, University of Stellenbosch
Disciplines: Not specified
Roles: Not specified
Expertise: Not specified
Tools: Not specified
Silicon cell model for the central carbohydrate metabolism of the archaeon Sulfolobus solfataricus under temperature variation
Programme: SysMO
Public web page: http://sulfosys.com/
Organisms: Sulfolobus solfataricus
An investigation in the CCM of S. solfataricus with a focus on the unique temperature adaptations and regulation; using a combined modelling and experimental approach.
Snapshots: Snapshot 1
Studies: Carbon loss at high T, Model gluconeogenesis, Pathway optimisation
Assays: Experimental assays of the temperature dependent degradation of GAP and ..., FBPA/ase, FBPAase model, GAPDH, GAPDH modeling, Gluconeogenic model, Model reconstituted system, Modelling pathway optimisation, Modelling temperature dependent degradation of GAP and DHAP, PGK, PGK and FBPAase titrations, PGK modeling, Reconstituted system reference state, TPI, TPI modeling
Scales: Not specified
Mathematical model of a subset of reactions comprising the three most temperature sensitive intermediates of the gluconeogenic pathway in S. solfataricus
Person responsible: Jacky Snoep
Snapshots: No snapshots
Investigation: Central Carbon Metabolism of Sulfolobus solfata...
Assays: Experimental assays of the temperature dependent degradation of GAP and ..., FBPA/ase, FBPAase model, GAPDH, GAPDH modeling, Gluconeogenic model, Modelling temperature dependent degradation of GAP and DHAP, PGK, PGK modeling, TPI, TPI modeling
Scales: Not specified
Contributor: Not available
Provider Name: Not specified
Provider's strain ID: Not specified
Organism: Sulfolobus solfataricus
Genotypes: wild-type
Phenotypes: wild-type
Comment: Not specified
The purified enzymes, PGK, GAPDH, TPI and FBPAase were incubated at 70 C en conversion of 3PG to F6P was followed
Scales: Not specified
The PGK, GAPDH, TPI and FBPAase were modelled together using the individual rate equations. Closed system.
Creator: Jacky Snoep
Contributor: Jacky Snoep
Model type: Ordinary differential equations (ODE)
Model format: SBML
Environment: JWS Online
Organism: Sulfolobus solfataricus
Investigations: Central Carbon Metabolism of Sulfolobus solfata...
Studies: Carbon loss at high T, Model gluconeogenesis, Pathway optimisation
Modelling analyses: Gluconeogenic model, Model reconstituted system, Modelling pathway optimisation
Scales: Not specified
Abstract (Expand)
Authors: Theresa Kouril, Bettina Siebers, Jacky Snoep, Esser D.,Kort J.,Westerhoff H. V.
Date Published: 22nd Aug 2013
Journal: FEBS J
PubMed ID: 23865479
Citation: FEBS J. 2013 Sep;280(18):4666-80. doi: 10.1111/febs.12438. Epub 2013 Aug 22.
Scales: Not specified