GAP and DHAP concentrations were measured over time at 70C.
Contributor: Jacky Snoep
Assay type: Experimental Assay Type
Technology type: Progressive Curve Experiment
Snapshots: No snapshots
Investigation: Central Carbon Metabolism of Sulfolobus solfata...
Study: Model gluconeogenesis
Organisms: No organisms
SOPs: No SOPs
Data files: T degradation DHAP, T degradation GAP
Kinetic characterization of the FBPA/ase with respect to GAP en DHAP saturation.
Technology type: Initial Rate Experiment
Organisms: Sulfolobus solfataricus : P2 (wild-type / wild-type)
SOPs: Expression of S. solfataricus enzymes in E. col..., Preparation of cell free extract of recombinant..., Protein purification from E. coli extracts.
Data files: FBPA/ase kinetics DHAP, FBPA/ase kinetics GAP, Model simulation and experimental data for DHAP...
Mathematical model for FBPAase kinetics, saturation of GAP and DHAP
Biological problem addressed: Model Analysis Type
Models: FBPAase kinetic model
Data files: GAPDH kinetics 3PG, GAPDH kinetics GAP, GAPDH kinetics NADP, GAPDH kinetics NADPH, GAPDH kinetics Pi
Mathematical model for GAPDH kinetics, saturation of GAP, BPG, NADP, NADPH and Pi.
Models: GAPDH kinetic model
Data files: GAPDH kinetics 3PG, GAPDH kinetics GAP, GAPDH kinetics NADP, GAPDH kinetics NADPH, GAPDH kinetics Pi and 1 hidden item
Mathematical model for the analysis of carbon loss of BPG, GAP and DHAP in reconstituted system of S. solfataricus at 70C.
Models: Mathematical model for the combined four enzyme...
Data files: Metabolite concentrations during reconstituted ...
The temperature dependent degradation of DHAP and GAP is modelled as an exponential decay process using experimental data for the disintegration at 70C.
Biological problem addressed: Metabolism
Models: DHAP T degradation, GAP T degradation
Data files: Model simulation and Exp data for DHAP T deg, Model simulation and Exp data for GAP T deg, T degradation DHAP, T degradation GAP
Kinetic characterisation of Phosphoglycerokinase
Data files: Model simulation and experimental data for ADP,..., PGK kinetics 3PG, PGK kinetics ADP, PGK kinetics ATP, PGK kinetics BPG
Mathematical model for PGK kinetics, saturation with ADP, ATP, 3PG and BPG.
Models: 1 hidden item
Data files: Model simulation and exp data for TIM kinetics,..., TPI kinetics DHAP, TPI kinetics GAP, TPI kinetics PEP inhibition GAP, TPI kinetics PEP inhibition of DHAP
Mathematical model for TPI kinetics, saturation of GAP and DHAP.
Models: TIM kinetic model