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Increasing the amount of FBPAase in the reconstituted system and measuring the 3PG and F6P fluxes.
SEEK DEMO ID: http://demo.seek4science.org/data_files/54?version=1
Filename: FBPAase_titration.xlsx
Format: Spreadsheet
Size: 30.3 KB

Views: 1047 Downloads: 51
Created: 3rd Feb 2013 at 11:05
Last updated: 28th May 2015 at 10:50
Last used: 25th Apr 2018 at 08:28

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Related items
Projects: SulfoSys
Institutions: University of Duisburg-Essen
Projects: PSYSMO, MOSES, SysMO DB, SysMO-LAB, SulfoSys
Institutions: Manchester Centre for Integrative Systems Biology, University of Manchester, University of Stellenbosch
Disciplines: Not specified
Roles: Not specified
Expertise: Not specified
Tools: Not specified
Silicon cell model for the central carbohydrate metabolism of the archaeon Sulfolobus solfataricus under temperature variation
Programme: SysMO
Public web page: http://sulfosys.com/
Organisms: Sulfolobus solfataricus
An investigation in the CCM of S. solfataricus with a focus on the unique temperature adaptations and regulation; using a combined modelling and experimental approach.
Snapshots: Snapshot 1
Studies: Carbon loss at high T, Model gluconeogenesis, Pathway optimisation
Assays: Experimental assays of the temperature dependent degradation of GAP and ..., FBPA/ase, FBPAase model, GAPDH, GAPDH modeling, Gluconeogenic model, Model reconstituted system, Modelling pathway optimisation, Modelling temperature dependent degradation of GAP and DHAP, PGK, PGK and FBPAase titrations, PGK modeling, Reconstituted system reference state, TPI, TPI modeling
Scales: Not specified
By varying the input or output flux via PGK and FBPAase manipulation respectively the pathway efficiency can be optimised.
Person responsible: Jacky Snoep
Snapshots: No snapshots
Scales: Not specified
Varying the relative amounts of PGK and FBPAase and analyse the 3PG uptake and F6P production rates.
Investigation: Central Carbon Metabolism of Sulfolobus solfata...
Study: Pathway optimisation
Organisms: Sulfolobus solfataricus : P2 (wild-type / wild-type)
Models: Mathematical model for the combined four enzyme...
SOPs: No SOPs
Data files: FBPAase titration of reconstituted system, Model simulation and experimental data for PGK ..., PGK titration of reconstituted system, Pathway efficiency for 3PG to F6P conversion as...
Scales: Not specified
Experimental analysis of pathway fluxes with varying PGK and FBPAase concentrations in the incubation assay.
Contributor: Jacky Snoep
Assay type: Extracellular Metabolite Concentration
Technology type: Progressive Curve Experiment
Snapshots: No snapshots
Investigation: Central Carbon Metabolism of Sulfolobus solfata...
Study: Pathway optimisation
Organisms: Sulfolobus solfataricus : P2 (wild-type / wild-type)
SOPs: No SOPs
Data files: FBPAase titration of reconstituted system, Model simulation and experimental data for PGK ..., PGK titration of reconstituted system
Scales: Not specified