Intracellular metabolite measurements used for validation of the gycerol synthesis pathway model
Creator: Jacky Snoep
Contributor: Katy Wolstencroft
Investigations: Glycerol Synthesis in Saccharomyces cerevisiae
Studies: Glycerol Synthesis Pathway
Assays: Glycerol Synthesis Model construction, Intracellular Metabolites
Contributor: Jacky Snoep
Investigations: Central Carbon Metabolism of Sulfolobus solfata...
Studies: Model gluconeogenesis
Assays: FBPA/ase, FBPAase model
Increasing the amount of FBPAase in the reconstituted system and measuring the 3PG and F6P fluxes.
Studies: Pathway optimisation
Assays: Modelling pathway optimisation, PGK and FBPAase titrations
Assays: GAPDH, GAPDH modeling
The purified enzymes, PGK, GAPDH, TPI and FBPAase were incubated at 70 C en conversion of 3PG to F6P was followed
Studies: Carbon loss at high T, Model gluconeogenesis
Assays: Gluconeogenic model, Model reconstituted system, Reconstituted system reference state
A time simulation of the mathematical model is shown together with the experimental data to which the model was fitted.
Assays: Modelling temperature dependent degradation of ...
Experimental data for the reconstituted system are plotted together with the model prediction
Studies: Carbon loss at high T
Assays: Model reconstituted system, Reconstituted system reference state
Model simulation and exp data for TIM kinetics, GAP and DHAP saturation, and PEP inhibition.
Assays: TPI, TPI modeling
Model simulation and experimental data for ADP, ATP, 3PG and BPG saturation of PGK
Assays: PGK, PGK modeling
Model simulation and experimental data for DHAP and GAP saturation of FBPA/ase
Combined plot fo the model simulations and the experimental data for the PGK and FBPAase titrations.
Investigations: Glycolitic Flux in Lactococcus lactis
Studies: The effects of Redox Charge on Glycolytic Flux
Assays: Time dependent responses of glycolytic intermed...
Saturation of PGK with 3PG
Kinetic characterisation of Phosphoglycerokinase. Measures enzyme reaction rate with increasing concentration of ATP
Increasing the amount of PGK in the incubation assay and measuring the 3PG and F6P fluxes.
Model simulation showing the effect of varying PGK or FBPAase concentration on the efficiency for 3PG to F6P conversion.
Assays: Modelling pathway optimisation
DHAP concentrations were measured over time at 70C.
Assays: Experimental assays of the temperature dependen..., Modelling temperature dependent degradation of ...
GAP concentrations were measured over time at 70C.