1. Home
  2. Data files Index
  3. Model simulation and experimental data for PGK and FBPAase titrations

Model simulation and experimental data for PGK and FBPAase titrations
Version 1

Download View content

Combined plot fo the model simulations and the experimental data for the PGK and FBPAase titrations.

SEEK ID: https://demo.fairdomhub.org/data_files/55?version=1

Filename: pgk-ald-titr.jpg  Download

Format: JPEG image

Size: 42.7 KB

Help
help Contributor and Creators
License
No license - no permission to use unless the owner grants a licence
Activity

Views: 3290   Downloads: 319

Created: 3rd Feb 2013 at 11:13

Last updated: 26th Oct 2017 at 11:57

Last used: 21st Jul 2019 at 17:06

help Attributions

None

help Scales
Not Specified

Related items

SulfoSys

Silicon cell model for the central carbohydrate metabolism of the archaeon Sulfolobus solfataricus under temperature variation

Programme: SysMO

Public web page: http://sulfosys.com/

Central Carbon Metabolism of Sulfolobus solfataricus
SulfoSys

An investigation in the CCM of S. solfataricus with a focus on the unique temperature adaptations and regulation; using a combined modelling and experimental approach.

Snapshots: Snapshot 1

Pathway optimisation
SulfoSys

By varying the input or output flux via PGK and FBPAase manipulation respectively the pathway efficiency can be optimised.

Person responsible: Jacky Snoep

Snapshots: No snapshots

Modelling pathway optimisation
SulfoSys

Varying the relative amounts of PGK and FBPAase and analyse the 3PG uptake and F6P production rates.

Contributor: Jacky Snoep

Biological problem addressed: Model Analysis Type

Snapshots: No snapshots

PGK and FBPAase titrations
SulfoSys

Experimental analysis of pathway fluxes with varying PGK and FBPAase concentrations in the incubation assay.

Contributor: Jacky Snoep

Assay type: Extracellular Metabolite Concentration

Technology type: Progressive Curve Experiment

Snapshots: No snapshots

Intermediate instability at high temperature leads to low pathway efficiency for an in vitro reconstituted system of gluconeogenesis in Sulfolobus solfataricus
SulfoSys

Abstract (Expand)

Four enzymes of the gluconeogenic pathway in Sulfolobus solfataricus were purified and kinetically characterized. The enzymes were reconstituted in vitro to quantify the contribution of temperature … instability of the pathway intermediates to carbon loss from the system. The reconstituted system, consisting of phosphoglycerate kinase, glyceraldehyde 3-phosphate dehydrogenase, triose phosphate isomerase and the fructose 1,6-bisphosphate aldolase/phosphatase, maintained a constant consumption rate of 3-phosphoglycerate and production of fructose 6-phosphate over a 1-h period. Cofactors ATP and NADPH were regenerated via pyruvate kinase and glucose dehydrogenase. A mathematical model was constructed on the basis of the kinetics of the purified enzymes and the measured half-life times of the pathway intermediates. The model quantitatively predicted the system fluxes and metabolite concentrations. Relative enzyme concentrations were chosen such that half the carbon in the system was lost due to degradation of the thermolabile intermediates dihydroxyacetone phosphate, glyceraldehyde 3-phosphate and 1,3-bisphosphoglycerate, indicating that intermediate instability at high temperature can significantly affect pathway efficiency.

Authors: T. Kouril, D. Esser, J. Kort, H. V. Westerhoff, B. Siebers, Jacky Snoep

Date Published: 22nd Aug 2013

Journal: FEBS J

Powered by
 (v.1.9.0)
FAIRDOM | Funding and Programmes | Credits | Imprint
Copyright © 2008 - 2019 The University of Manchester and HITS gGmbH