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Experimental analysis of pathway fluxes with varying PGK and FBPAase concentrations in the incubation assay.
SEEK ID: https://demo.fairdomhub.org/assays/30
Experimental Assay
Projects: SulfoSys
Investigation: Central Carbon Metabolism of Sulfolobus solfataricus
Study: Pathway optimisation
Assay type: Extracellular Metabolite Concentration
Technology type: Progressive Curve Experiment
Organisms: Sulfolobus solfataricus : P2 (wild-type / wild-type)
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Created: 3rd Feb 2013 at 10:18
Last updated: 26th Oct 2017 at 09:57

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Silicon cell model for the central carbohydrate metabolism of the archaeon Sulfolobus solfataricus under temperature variation
Programme: SysMO
Public web page: http://sulfosys.com/
Organisms: Sulfolobus solfataricus
An investigation in the CCM of S. solfataricus with a focus on the unique temperature adaptations and regulation; using a combined modelling and experimental approach.
Snapshots: Snapshot 1
Studies: Carbon loss at high T, Model gluconeogenesis, Pathway optimisation
Assays: Experimental assays of the temperature dependent degradation of GAP and ..., FBPA/ase, FBPAase model, GAPDH, GAPDH modeling, Gluconeogenic model, Model reconstituted system, Modelling pathway optimisation, Modelling temperature dependent degradation of GAP and DHAP, PGK, PGK and FBPAase titrations, PGK modeling, Reconstituted system reference state, TPI, TPI modeling
Scales: Not specified
By varying the input or output flux via PGK and FBPAase manipulation respectively the pathway efficiency can be optimised.
Person responsible: Jacky Snoep
Snapshots: No snapshots
Scales: Not specified
Submitter: Not available
Provider Name: Not specified
Provider's strain ID: Not specified
Organism: Sulfolobus solfataricus
Genotypes: wild-type
Phenotypes: wild-type
Comment: Not specified
Combined plot fo the model simulations and the experimental data for the PGK and FBPAase titrations.
Investigations: Central Carbon Metabolism of Sulfolobus solfata...
Studies: Pathway optimisation
Assays: Modelling pathway optimisation, PGK and FBPAase titrations
Scales: Not specified
Increasing the amount of PGK in the incubation assay and measuring the 3PG and F6P fluxes.
Investigations: Central Carbon Metabolism of Sulfolobus solfata...
Studies: Pathway optimisation
Assays: Modelling pathway optimisation, PGK and FBPAase titrations
Scales: Not specified
Increasing the amount of FBPAase in the reconstituted system and measuring the 3PG and F6P fluxes.
Investigations: Central Carbon Metabolism of Sulfolobus solfata...
Studies: Pathway optimisation
Assays: Modelling pathway optimisation, PGK and FBPAase titrations
Scales: Not specified
Abstract (Expand)
Authors: T. Kouril, D. Esser, J. Kort, H. V. Westerhoff, B. Siebers, J. L. Snoep
Date Published: 22nd Aug 2013
Publication Type: Not specified
PubMed ID: 23865479
Citation: FEBS J. 2013 Sep;280(18):4666-80. doi: 10.1111/febs.12438. Epub 2013 Aug 22.
Scales: Not specified