PGK and FBPAase titrations
Experimental analysis of pathway fluxes with varying PGK and FBPAase concentrations in the incubation assay.
SEEK ID: https://demo.fairdomhub.org/assays/30
Experimental Assay
Projects: SulfoSys
Investigation: Central Carbon Metabolism of Sulfolobus solfataricus
Study: Pathway optimisation
Assay type: Extracellular Metabolite Concentration
Technology type: Progressive Curve Experiment
Organisms: Sulfolobus solfataricus : P2 (wild-type / wild-type)
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Created: 3rd Feb 2013 at 11:18
Last updated: 26th Oct 2017 at 11:57
ScalesRelated items
Silicon cell model for the central carbohydrate metabolism of the archaeon Sulfolobus solfataricus under temperature variation
Programme: SysMO
Public web page: http://sulfosys.com/
An investigation in the CCM of S. solfataricus with a focus on the unique temperature adaptations and regulation; using a combined modelling and experimental approach.
Snapshots: Snapshot 1
Scales: Not specified
By varying the input or output flux via PGK and FBPAase manipulation respectively the pathway efficiency can be optimised.
Person responsible: Jacky Snoep
Snapshots: No snapshots
Scales: Not specified
Contributor: Not available
Provider Name: Not specified
Provider's strain ID: Not specified
Organism: Sulfolobus solfataricus
Genotypes: wild-type
Phenotypes: wild-type
Comment: Not specified
Combined plot fo the model simulations and the experimental data for the PGK and FBPAase titrations.
Scales: Not specified
Increasing the amount of PGK in the incubation assay and measuring the 3PG and F6P fluxes.
Scales: Not specified
Increasing the amount of FBPAase in the reconstituted system and measuring the 3PG and F6P fluxes.
Scales: Not specified
Abstract (Expand)
Authors: T. Kouril, D. Esser, J. Kort, H. V. Westerhoff, B. Siebers, Jacky Snoep
Date Published: 22nd Aug 2013
Journal: FEBS J
Scales: Not specified
