Assays
What is an Assay?A kinetic model of the glycerol synthesis pathway has been constructed. Kinetic parameters were collected from published values. Maximal enzyme activities and intracellular effector concentrations were determined experimentally.
Submitter: Katy Wolstencroft
Biological problem addressed: Metabolism
Investigation: Glycerol Synthesis in Saccharomyces cerevisiae
Study: Glycerol Synthesis Pathway
Intracellular metabolite concentrations were determined enzymatically by measuring the oxidation or reduction of NADH or NAD+, respectively, at 340 nm in a Beckman Coulter DU640 spectrophotometer
Submitter: Katy Wolstencroft
Assay type: Intracellular Metabolite Concentration
Technology type: Enzymatic Activity Measurements
Investigation: Glycerol Synthesis in Saccharomyces cerevisiae
Study: Glycerol Synthesis Pathway