Intracellular metabolite measurements used for validation of the gycerol synthesis pathway model
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Created: 19th Aug 2011 at 11:30
Last updated: 4th Nov 2011 at 09:32
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Version 1 (earliest) Created 19th Aug 2011 at 11:30 by Katy Wolstencroft
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Projects: SysMO-LAB, PSYSMO, MOSES, SulfoSys, SysMO DB
Institutions: Manchester Centre for Integrative Systems Biology, University of Manchester, University of Stellenbosch
Expertise: Biochemistry, Bioinformatics, Data Management
Tools: Data Management, Transcriptomics, Databases, Workflows, Web services, Taverna, Ontologies
I am a Research Fellow at the University of Manchester, working in Bioinformatics and Computer Science. I am also a guest researcher at the Vrije Universiteit.My research interests include scientific workflows, semantic discovery, and applying ontology technologies to biological data
SysMO is a European transnational funding and research initiative on "Systems Biology of Microorganisms".
The goal pursued by SysMO is to record and describe the dynamic molecular processes going on in unicellular microorganisms in a comprehensive way and to present these processes in the form of computerized mathematical models.
Systems biology will raise biomedical and biotechnological research to a new quality level and contribute markedly to progress in understanding. Pooling European research ...
Projects: BaCell-SysMO, COSMIC, SUMO, KOSMOBAC, SysMO-LAB, PSYSMO, SCaRAB, MOSES, TRANSLUCENT, STREAM, SulfoSys, SysMO DB
Web page: http://sysmo.net/
The main objectives of SysMO-DB are to: facilitate the web-based exchange of data between research groups within- and inter- consortia, and to provide an integrated platform for the dissemination of the results of the SysMO projects to the scientific community. We aim to devise a progressive and scalable solution to the data management needs of the SysMO initiative, that:
- facilitates and maximises the potential for data exchange between SysMO research groups;
- maximises the ‘shelf life’ and ...
Programme: SysMO
Public web page: http://www.sysmo-db.org/
Organisms: Saccharomyces cerevisiae, Lactic Acid Bacteria
Glycerol, a major by-product of ethanol fermentation by Saccharomyces cerevisiae, is of significant importance to the wine, beer, and ethanol production industries. This investigation aims to gain a clearer understanding of and to quantify the extent to which parameters of the pathway affect glycerol flux in S. cerevisiae.
This investigation represents the data and models submitted to JWS online by Cronwright et al (http://jjj.biochem.sun.ac.za/cgi-bin/getMoreInfo.py?modeldir=cronwright) and ...
Submitter: Katy Wolstencroft
Studies: Glycerol Synthesis Pathway
Assays: Extracellular Glycerol, Glycerol Synthesis Model construction, Intracellular Metabolites
Snapshots: No snapshots
This is a study of the glycerol synthesis pathway, using model predictions and experimental measurements
Submitter: Katy Wolstencroft
Investigation: Glycerol Synthesis in Saccharomyces cerevisiae
Assays: Extracellular Glycerol, Glycerol Synthesis Model construction, Intracellular Metabolites
Snapshots: No snapshots
A kinetic model of the glycerol synthesis pathway has been constructed. Kinetic parameters were collected from published values. Maximal enzyme activities and intracellular effector concentrations were determined experimentally.
Submitter: Katy Wolstencroft
Biological problem addressed: Metabolism
Investigation: Glycerol Synthesis in Saccharomyces cerevisiae
Study: Glycerol Synthesis Pathway
Organisms: Saccharomyces cerevisiae
Models: Metabolic Control Analysis of Glycerol Synthesi...
SOPs: No SOPs
Data files: Cronwright Parameter Data
Snapshots: No snapshots
Intracellular metabolite concentrations were determined enzymatically by measuring the oxidation or reduction of NADH or NAD+, respectively, at 340 nm in a Beckman Coulter DU640 spectrophotometer
Submitter: Katy Wolstencroft
Assay type: Intracellular Metabolite Concentration
Technology type: Enzymatic Activity Measurements
Investigation: Glycerol Synthesis in Saccharomyces cerevisiae
Study: Glycerol Synthesis Pathway
Organisms: Saccharomyces cerevisiae
SOPs: No SOPs
Data files: Cronwright Parameter Data
Snapshots: No snapshots