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Model simulation and experimental data for ADP, ATP, 3PG and BPG saturation of PGK

Model simulation and exp data for TIM kinetics, GAP and DHAP saturation, and PEP inhibition.

Experimental data for the reconstituted system are plotted together with the model prediction

Increasing the amount of PGK in the incubation assay and measuring the 3PG and F6P fluxes.

The purified enzymes, PGK, GAPDH, TPI and FBPAase were incubated at 70 C en conversion of 3PG to F6P was followed

Increasing the amount of FBPAase in the reconstituted system and measuring the 3PG and F6P fluxes.

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