ExploreIncreasing the amount of PGK in the incubation assay and measuring the 3PG and F6P fluxes.
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PGK_titration.xlsx
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Contributor and Creators
Views: 1841 Downloads: 142
Created: 3rd Feb 2013 at 11:03
Last updated: 28th May 2015 at 10:50
Last used: 20th Nov 2018 at 16:57
AttributionsNone
ScalesRelated items
Projects: PSYSMO, MOSES, SysMO DB, SysMO-LAB, SulfoSys
Institutions: Manchester Centre for Integrative Systems Biology, University of Manchester, University of Stellenbosch
Silicon cell model for the central carbohydrate metabolism of the archaeon Sulfolobus solfataricus under temperature variation
Programme: SysMO
Public web page: http://sulfosys.com/
An investigation in the CCM of S. solfataricus with a focus on the unique temperature adaptations and regulation; using a combined modelling and experimental approach.
Snapshots: Snapshot 1
Scales: Not specified
By varying the input or output flux via PGK and FBPAase manipulation respectively the pathway efficiency can be optimised.
Person responsible: Jacky Snoep
Snapshots: No snapshots
Scales: Not specified
Varying the relative amounts of PGK and FBPAase and analyse the 3PG uptake and F6P production rates.
Scales: Not specified
Experimental analysis of pathway fluxes with varying PGK and FBPAase concentrations in the incubation assay.
Contributor: Jacky Snoep
Assay type: Extracellular Metabolite Concentration
Technology type: Progressive Curve Experiment
Snapshots: No snapshots
Scales: Not specified
