Model simulation showing the effect of varying PGK or FBPAase concentration on the efficiency for 3PG to F6P conversion.
Creator: Jacky Snoep
Contributor: Jacky Snoep
Investigations: Central Carbon Metabolism of Sulfolobus solfata...
Studies: Pathway optimisation
Assays: Modelling pathway optimisation
Saturation of PGK with 3PG
Studies: Model gluconeogenesis
Assays: PGK, PGK modeling
Kinetic characterisation of Phosphoglycerokinase. Measures enzyme reaction rate with increasing concentration of ATP
Increasing the amount of PGK in the incubation assay and measuring the 3PG and F6P fluxes.
Assays: Modelling pathway optimisation, PGK and FBPAase titrations