Data files

results of metabolic profiling of Saccharomyces cerevisiae in different conditions

Results of transcriptional profile in Saccharomyces cerevisiae after rapamycin treatment

Results of proteomics profile in Saccharomyces cerevisiae under different experimental conditions

Results of transcriptional profile in saccharomyces cerevisiae under different experimental conditions

Intracellular metabolite measurements used for validation of the gycerol synthesis pathway model

Increasing the amount of FBPAase in the reconstituted system and measuring the 3PG and F6P fluxes.

The purified enzymes, PGK, GAPDH, TPI and FBPAase were incubated at 70 C en conversion of 3PG to F6P was followed

A time simulation of the mathematical model is shown together with the experimental data to which the model was fitted.

A time simulation of the mathematical model is shown together with the experimental data to which the model was fitted.

Experimental data for the reconstituted system are plotted together with the model prediction

Model simulation and exp data for TIM kinetics, GAP and DHAP saturation, and PEP inhibition.

Model simulation and experimental data for ADP, ATP, 3PG and BPG saturation of PGK

Model simulation and experimental data for DHAP and GAP saturation of FBPA/ase

Combined plot fo the model simulations and the experimental data for the PGK and FBPAase titrations.

File to use as an example for OpenRefine computer paractical

Model simulation showing the effect of varying PGK or FBPAase concentration on the efficiency for 3PG to F6P conversion.

Saturation of PGK with 3PG

Kinetic characterisation of Phosphoglycerokinase. Measures enzyme reaction rate with increasing concentration of ATP

Increasing the amount of PGK in the incubation assay and measuring the 3PG and F6P fluxes.

DHAP concentrations were measured over time at 70C.

GAP concentrations were measured over time at 70C.