Technology type 'Progressive Curve Experiment'

Enzymatic Activity Measurements
Current technology type

Progressive Curve Experiment
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Related assays

3 Assays visible to you, out of a total of 3

Experimental assays of the temperature dependent degradation of GAP and DHAP

SulfoSys

GAP and DHAP concentrations were measured over time at 70C.

Submitter: Jacky Snoep

Assay type: Experimental Assay Type

Technology type: Progressive Curve Experiment

Investigation: Central Carbon Metabolism of Sulfolobus solfata...

Study: Model gluconeogenesis

Organisms: No organisms

SOPs: No SOPs

Data files: T degradation DHAP, T degradation GAP

Snapshots: No snapshots

Created: 29th Jan 2013 at 21:01, Last updated: 26th Oct 2017 at 09:57

Reconstituted system reference state

SulfoSys

The four purified enzymes were incubated in assay buffer and consumption of 3PG and production of F6P were measured in time, together with GAP and DHAP concentrations.

Submitter: Jacky Snoep

Assay type: Experimental Assay Type

Technology type: Progressive Curve Experiment

Investigation: Central Carbon Metabolism of Sulfolobus solfata...

Study: Carbon loss at high T

Organisms: Sulfolobus solfataricus : P2 (wild-type / wild-type)

SOPs: No SOPs

Data files: Metabolite concentrations during reconstituted ..., Model simulation and Exp data for reconstituted...

Snapshots: No snapshots

Created: 2nd Feb 2013 at 16:06, Last updated: 26th Oct 2017 at 09:57

PGK and FBPAase titrations

SulfoSys

Experimental analysis of pathway fluxes with varying PGK and FBPAase concentrations in the incubation assay.

Submitter: Jacky Snoep

Assay type: Extracellular Metabolite Concentration

Technology type: Progressive Curve Experiment

Investigation: Central Carbon Metabolism of Sulfolobus solfata...

Study: Pathway optimisation

Organisms: Sulfolobus solfataricus : P2 (wild-type / wild-type)

SOPs: No SOPs

Data files: FBPAase titration of reconstituted system, Model simulation and experimental data for PGK ..., PGK titration of reconstituted system

Snapshots: No snapshots

Created: 3rd Feb 2013 at 10:18, Last updated: 26th Oct 2017 at 09:57