Technology type 'Enzymatic Activity Measurements'

Technology Type
Current technology type

Enzymatic Activity Measurements
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Initial Rate Experiment (4) | Progressive Curve Experiment (3)

Related assays

8 Assays visible to you, out of a total of 8

Intracellular Metabolites

SysMO DB

Intracellular metabolite concentrations were determined enzymatically by measuring the oxidation or reduction of NADH or NAD+, respectively, at 340 nm in a Beckman Coulter DU640 spectrophotometer

Submitter: Katy Wolstencroft

Assay type: Intracellular Metabolite Concentration

Technology type: Enzymatic Activity Measurements

Investigation: Glycerol Synthesis in Saccharomyces cerevisiae

Study: Glycerol Synthesis Pathway

Organisms: Saccharomyces cerevisiae

SOPs: No SOPs

Data files: Cronwright Parameter Data

Snapshots: No snapshots

Created: 19th Aug 2011 at 11:28, Last updated: 26th Oct 2017 at 09:57

PGK

SulfoSys

Kinetic characterisation of Phosphoglycerokinase

Submitter: Jacky Snoep

Assay type: Experimental Assay Type

Technology type: Initial Rate Experiment

Investigation: Central Carbon Metabolism of Sulfolobus solfata...

Study: Model gluconeogenesis

Organisms: Sulfolobus solfataricus : P2 (wild-type / wild-type)

SOPs: Expression of S. solfataricus enzymes in E. col..., Preparation of cell free extract of recombinant..., Protein purification from E. coli extracts.

Data files: Model simulation and experimental data for ADP,..., PGK kinetics 3PG, PGK kinetics ADP, PGK kinetics ATP, PGK kinetics BPG

Snapshots: No snapshots

Created: 29th Jan 2013 at 14:18, Last updated: 26th Oct 2017 at 09:57

GAPDH

SulfoSys

No description specified

Submitter: Jacky Snoep

Assay type: Experimental Assay Type

Technology type: Initial Rate Experiment

Investigation: Central Carbon Metabolism of Sulfolobus solfata...

Study: Model gluconeogenesis

Organisms: Sulfolobus solfataricus : P2 (wild-type / wild-type)

SOPs: Expression of S. solfataricus enzymes in E. col..., Preparation of cell free extract of recombinant..., Protein purification from E. coli extracts.

Data files: GAPDH kinetics 3PG, GAPDH kinetics GAP, GAPDH kinetics NADP, GAPDH kinetics NADPH, GAPDH kinetics Pi

Snapshots: No snapshots

Created: 29th Jan 2013 at 20:37, Last updated: 26th Oct 2017 at 09:57

TPI

SulfoSys

No description specified

Submitter: Jacky Snoep

Assay type: Experimental Assay Type

Technology type: Initial Rate Experiment

Investigation: Central Carbon Metabolism of Sulfolobus solfata...

Study: Model gluconeogenesis

Organisms: Sulfolobus solfataricus : P2 (wild-type / wild-type)

SOPs: Expression of S. solfataricus enzymes in E. col..., Preparation of cell free extract of recombinant..., Protein purification from E. coli extracts.

Data files: Model simulation and exp data for TIM kinetics,..., TPI kinetics DHAP, TPI kinetics GAP, TPI kinetics PEP inhibition GAP, TPI kinetics PEP inhibition of DHAP

Snapshots: No snapshots

Created: 29th Jan 2013 at 20:45, Last updated: 26th Oct 2017 at 09:57

FBPA/ase

SulfoSys

Kinetic characterization of the FBPA/ase with respect to GAP en DHAP saturation.

Submitter: Jacky Snoep

Assay type: Experimental Assay Type

Technology type: Initial Rate Experiment

Investigation: Central Carbon Metabolism of Sulfolobus solfata...

Study: Model gluconeogenesis

Organisms: Sulfolobus solfataricus : P2 (wild-type / wild-type)

SOPs: Expression of S. solfataricus enzymes in E. col..., Preparation of cell free extract of recombinant..., Protein purification from E. coli extracts.

Data files: FBPA/ase kinetics DHAP, FBPA/ase kinetics GAP, Model simulation and experimental data for DHAP...

Snapshots: No snapshots

Created: 29th Jan 2013 at 20:51, Last updated: 26th Oct 2017 at 09:57

Experimental assays of the temperature dependent degradation of GAP and DHAP

SulfoSys

GAP and DHAP concentrations were measured over time at 70C.

Submitter: Jacky Snoep

Assay type: Experimental Assay Type

Technology type: Progressive Curve Experiment

Investigation: Central Carbon Metabolism of Sulfolobus solfata...

Study: Model gluconeogenesis

Organisms: No organisms

SOPs: No SOPs

Data files: T degradation DHAP, T degradation GAP

Snapshots: No snapshots

Created: 29th Jan 2013 at 21:01, Last updated: 26th Oct 2017 at 09:57

Reconstituted system reference state

SulfoSys

The four purified enzymes were incubated in assay buffer and consumption of 3PG and production of F6P were measured in time, together with GAP and DHAP concentrations.

Submitter: Jacky Snoep

Assay type: Experimental Assay Type

Technology type: Progressive Curve Experiment

Investigation: Central Carbon Metabolism of Sulfolobus solfata...

Study: Carbon loss at high T

Organisms: Sulfolobus solfataricus : P2 (wild-type / wild-type)

SOPs: No SOPs

Data files: Metabolite concentrations during reconstituted ..., Model simulation and Exp data for reconstituted...

Snapshots: No snapshots

Created: 2nd Feb 2013 at 16:06, Last updated: 26th Oct 2017 at 09:57

PGK and FBPAase titrations